What is the difference between Fast and Standard mode on the AB Real-Time PCR Instruments?
Most instruments have the ability to run in either Fast or Standard mode. When compared to a Standard mode run, a Fast run uses smaller reaction volumes and faster ramp rates. This results in a Fast run taking only about 40 min to complete, while a Standard mode run takes about 2 hrs. In addition to a shorter run time, a Fast run has the benefits of requiring less reagents (due to smaller reaction volumes), which saves money. However sometimes a Standard run is required due the assay type, or the need to use larger reaction volumes (such as environmental samples). Lastly, a Fast run must be completed on a Fast block and must use the appropriate consumables/plate types.
Here are the typical reaction input volumes for different blocks. Also notice the differences in the plate types in the image below:
• 96-well Standard: 25-100 µL
• 96-well Fast: 10-30 µL