Routine maintenance of Real-Time PCR instruments is important to keep them operating optimally. Every instrument has a recommended maintenance schedule, which for our Real-Time instruments includes a monthly background calibration. The background calibration checks to make sure that the block has not become contaminated, which could affect your data.
Thankfully the background calibration is quick ( < 15 min) and easy to do. If the background calibration would happen to fail, it is simple to clean the block and retry. Check out the short video below for a demonstration of the cleaning process using the 7500. If you have a newer instrument such as the Viia7 or QuantStudio, cleaning the block is even easier, as the block can be removed for easy access.